Journal: Materials Today Bio

Article Title: 3D-printed PRP-infused double-network hydrogels orchestrate inflammation resolution and vascular regeneration in infected wounds

doi: 10.1016/j.mtbio.2026.102841

Figure Lengend Snippet: In vitro Anti-inflammatory ability of hydrogels by driving macrophage polarization. (A) Immunofluorescence images of the M1 and M2-type macrophages in different groups. (B, C) Quantitative analysis of positive cells (CD86 and CD206) fluorescence intensity values. (D) Western blot analysis for the expression of inflammation proteins (Arg-1 and iNOS) in RAW264.7 with hydrogels treatments, and (E, F) Semiquantitative analysis of corresponding protein expression levels. (G–I) ELISA analysis of specific markers of M1 macrophage and M2 macrophage with various treatments. (mean ± SD, n = 3, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001).

Article Snippet: The amount of growth factors released from the hydrogel was quantified using the QuantiCyto® Rat EGF ELISA Kit (Neobioscience Technology Co, Ltd.), Rat PDGF-A ELISA kit, and Rat VEGF ELISA Kit (CUSABIO, https://www.cusabio.com/ ).

Techniques: In Vitro, Immunofluorescence, Fluorescence, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Wound Repair and Regeneration

Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes

doi: 10.1111/wrr.70153

Figure Lengend Snippet: Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification of PDGF‐BB (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.

Article Snippet: The concentrations of platelet‐derived growth factor‐BB (PDGF‐BB) (E‐EL‐H1577, Elabscience, USA), P‐selectin (SEA569Hu; Cloud‐Clone Corp, USA) and CCL‐5 (Proteintech, USA) were determined using sandwich ELISA assays and the absorbance read at 450 nm using a microplate reader (Multiskan GO 1.00.40; Thermo‐Scientific Group, USA) according to the manufacturer's instructions.

Techniques: Comparison, Control